antibody

Anti-Eph receptor A2/EPHA2 Antibody

PA1744

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Enabled

Anti-Eph receptor A2/EPHA2 Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

EPHA2

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for ECK/EPHA2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. ECK/EPHA2 information: Molecular Weight: 108266 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single- pass type I membrane protein. Cell junction, focal adhesion. Present at regions of cell-cell contacts but also at the leading edge of migrating cells; Tissue Specificity: Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.

Rabbit IgG polyclonal antibody for Ephrin type-A receptor 2(EPHA2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

P29317

Rabbit

A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A2(954-976aa HQKRIAYSLLGLKDQVNTVGIPI), identical to the related rat and mouse sequences.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.

IHC, WB

Human, Mouse, Rat

Hamster

Anti-Eph receptor A2 antibody, PA1744, Western blotting. Lane 1: Rat Ovary Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: COLO320 Cell Lysate. Anti-Eph receptor A2 antibody, PA1744, IHC(P). IHC(P): Human Lung Cancer Tissue

EPHA2(ephrin type-A receptor 2) also known as ECK, is a protein that in humans is encoded by the EPHA2 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. By somatic cell hybrid analysis and fluorescence in situ hybridization, the EPHA2 gene is mapped to chromosome 1p36.1. By screening a HeLa cell cDNA library with degenerate oligonucleotides based on highly conserved regions of receptor protein-tyrosine kinases, Lindberg and Hunter isolated cDNAs encoding EPHA2, which they called ECK. EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry. EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1(CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.

sc 924 sc 31254 sc 101377 sc 10746 sc 31255 sc 135658 sc 398832

Angiogenesis, Axonal Guidance Proteins, Cardiovascular, Cell Type Marker, Growth And Development, Intracellular Signaling, Kinases, Neurology Process, Neuron Marker, Neuroscience, Neurotransmission, Receptors / Channels

Ephrin type-A receptor 2;2.7.10.1;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK;EPHA2;ECK;

Ephrin type-A receptor 2

108266 MW

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand- independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single- pass type I membrane protein. Cell junction, focal adhesion. Present at regions of cell-cell contacts but also at the leading edge of migrating cells.

Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.

Ephrin type-A receptor 2

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

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