antibody

Anti-CD146/MCAM Antibody

PA1736

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Enabled

Anti-CD146/MCAM Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

MCAM

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for CD146/MCAM detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. CD146/MCAM information: Molecular Weight: 71607 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Rabbit IgG polyclonal antibody for Cell surface glycoprotein MUC18(MCAM) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

P43121

Rabbit

A synthetic peptide corresponding to a sequence at the C-terminus of human CD146(584-600aa KKGKLPCRRSGKQEITL), different from the related rat and mouse sequences by one amino acid.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat.

IHC, WB

Human, Mouse, Rat

Horse, Monkey, Rabbit

Anti-CD146 antibody, PA1736, Western blotting. Lane 1: A549 Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: HELA Cell Lysate. Lane 4: SW620 Cell Lysate. Anti-CD146 antibody, PA1736, IHC(P). IHC(P): Human Intestinal Cancer Tissue

CD146(cluster of differentiation 146) also known as the melanoma cell adhesion molecule(MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. By radiation hybrid analysis, Kuske and Johnson(1999) mapped the CD146 gene to chromosome 11q23.3. It is a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells. CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency; mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface.

sc 135987 sc 18837 sc 18940 sc 18942 sc 28667 sc 374414 sc 374556 sc 376762 sc 53369 sc 71564 sc 71565

Cancer, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton / Ecm, Immunology, Invasion/Microenvironment, Signal Transduction, Stem Cells

Cell surface glycoprotein MUC18;Cell surface glycoprotein P1H12;Melanoma cell adhesion molecule;Melanoma-associated antigen A32;Melanoma-associated antigen MUC18;S-endo 1 endothelial-associated antigen;CD146;MCAM;MUC18;

Cell surface glycoprotein MUC18

71607 MW

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Membrane; Single-pass type I membrane protein.

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Cell surface glycoprotein MUC18

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

1/28/19 19:39