Anti-SDHA Antibody | Boster PA1717 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-SDHA Antibody

catalog :

PA1717

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

PA1717

Product Name :

Anti-SDHA Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Monkey, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Monkey, Mouse, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-SDHA Antibody catalog # PA1717. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

SDHA

Uniprot ID :

P31040

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human SDHA (113-128aa NMEEDNWRWHFYDTVK), identical to the related rat and mouse sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Synonyms :

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; 1.3.5.1; Flavoprotein subunit of complex II; Fp; SDHA; SDH2, SDHF;

Protein Name :

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Molecular Weight :

72692 MW

Protein Function :

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.

Subcellular Localization :

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Background :

SDHA (Succinate dehydrogenase complex, subunit a, flavoprotein), also called SDH1, HOMOLOG OF, is a protein that in humans is encoded by the SDHA gene. The SDHA gene is mapped on 5p15.33. The SDHA gene is highly polymorphic. The gene that codes for the SDHA protein is nuclear, even though the protein is located in the inner membrane of the mitochondria. Heterozygous carriers of an SDHA mutation do not developparagangliomas as has been seen for mutations in the other subunits. In vitro functional expression studies in the yeast homolog showed that the mutation resulted in a loss of SDH activity and rendered the mutant SDHA protein more susceptible to proteolysis. The findings indicated that SDHA, like other SDH subunits, can act as a tumor suppressor gene.

Research Category :

Mitochondria, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Organelles, Oxidative Phosphorylation, Oxidative Stress, Pathways And Processes, Redox Metabolism, Signal Transduction, Subcellular Markers, Tags & Cell Markers