antibody

Anti-JAK2 Antibody

PA1629

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Enabled

Anti-JAK2 Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

JAK2

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for JAK2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. JAK2 information: Molecular Weight: 130674 MW; Subcellular Localization: Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus ; Tissue Specificity: Ubiquitously expressed throughout most tissues.

Rabbit IgG polyclonal antibody for Tyrosine-protein kinase JAK2(JAK2) detection. Tested with WB, IHC-P, ICC/IF in Human.

100 ug/vial

30ug for $99, contact us for details

O60674

Rabbit

A synthetic peptide corresponding to a sequence at the C-terminus of human JAK2(1096-1113aa DEIYMIMTECWNNNVNQR).

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunocytochemistry/Immunofluorescence, 2ug/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Western blot, 0.1-0.5ug/ml, Human.

IF, IHC-P, ICC, WB

Human

Hamster

Figure 1. Western blot analysis of JAK2 using anti-JAK2 antibody (PA1629). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, . Lane 2: rat H9C2 whole cell lysates, . Lane 3: human PC-3 whole cell lysates, . Lane 4: human SW620 whole cell lysates,. Lane 5: human Jurkat whole cell lysates,. Lane 6: rat PC-12 whole cell lysates,. Lane 7: rat NRK whole cell lysates,. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK2 antigen affinity purified polyclonal antibody (Catalog # PA1629) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK2 at approximately 130-140KD. The expected band size for JAK2 is at 130KD. Figure 2. IHC analysis of JAK2 using anti- JAK2 antibody ( PA1629). JAK2 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- JAK2 Antibody ( PA1629) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of JAK2 using anti- JAK2 antibody ( PA1629). JAK2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- JAK2 Antibody ( PA1629) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Janus kinase 2, commanly called JAK2, is a human protein that has been implicated in signaling by members of the type II cytokine receptor family, the GM-CSF receptor family, the gp130 receptor family, and the single chain receptors. Exactly, JAK2 kinase is a member of a family of tyrosine kinases involved in cytokine receptor signaling. The JAK2 gene is mapped to 9p24.1. The JAK2 gene encodes a 1,132-amino acid protein that shares 95% sequence similarity to rat and pig Jak2. JAK2 is constitutively associated with the prolactin receptor and that it is activated and tyrosine phosphorylated upon PRL binding to the PRL receptor. JAK2, and more specifically just its intact N-terminal domain, binds to EPOR in the endoplasmic reticulum and promotes its cell surface expression. The human JAK2 is present in the nucleus of hematopoietic cells and directly phosphorylates tyr41 on histone H3.

sc 34480 sc 278 sc 294 sc 34479 sc 7229 sc 390539 sc 294 G sc 34479 R

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics And Nuclear Signaling, Kinases/Phosphatases, Nuclear Signaling Pathways, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Signal Transduction, Tyrosine Kinases

Tyrosine-protein kinase JAK2;2.7.10.2;Janus kinase 2;JAK-2;JAK2;

Tyrosine-protein kinase JAK2

130674 MW

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus .

Ubiquitously expressed throughout most tissues.

Tyrosine-protein kinase JAK2

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

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