Anti-Heat shock factor protein 2 HSF2 Antibody | Boster PA1607 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Heat shock factor protein 2 HSF2 Antibody

catalog :

PA1607

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

PA1607

Product Name :

Anti-Heat shock factor protein 2 HSF2 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Rat

Predicted Reactivity :

Hamster

Application(s) :

Flow Cytometry, IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Rat, By Heat. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Heat shock factor protein 2 HSF2 Antibody catalog # PA1607. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

HSF2

Uniprot ID :

Q03933

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human HSF2 (82-102aa KQERDGPVEFQHPYFKQGQDD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Heat shock factor protein 2

Synonyms :

Heat shock factor protein 2; HSF 2; Heat shock transcription factor 2; HSTF 2; HSF2; HSTF2;

Protein Name :

Heat shock factor protein 2(HSF 2)

Molecular Weight :

60348 MW

Protein Function :

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Subcellular Localization :

Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Sequence Similarities :

Belongs to the HSF family.

Background :

HSF2 (HEAT-SHOCK TRANSCRIPTION FACTOR 2), also called HEAT-SHOCK FACTOR 2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. The International Radiation Hybrid Mapping Consortium maps the HSF2 gene to chromosome 6. Mouse Hsf2 was expressed in all 3 embryonic layers at embryonic day 7.5 and that the head fold was strongly stained at embryonic day 8.5. In adults, Hsf2 expression is detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells. Hsp70i bookmarking is mediated by a transcription factor called HSF2, which binds this promoter in mitotic cells, recruits protein phosphatase-2A, and interacts with the CAPG subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. And Hsf2-null mice were born at the expected mendelian ratio but had brain abnormalities and meiotic and gametogenic defects in both genders.

Research Category :

Epigenetics and Nuclear Signaling, Transcription