Anti-GRB2 Antibody | Boster PA1579 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GRB2 Antibody

catalog :

PA1579

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

sku :

PA1579

status :

Enabled

name :

Anti-GRB2 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

GRB2

price various sizes :

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for GRB2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. GRB2 information: Molecular Weight: 25206 MW; Subcellular Localization: Nucleus . Cytoplasm . Endosome . Golgi apparatus .

short description :

Rabbit IgG polyclonal antibody for Growth factor receptor-bound protein 2(GRB2) detection. Tested with WB in Human;Mouse;Rat.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

P62993

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human GRB2(184-201aa HVMDNSDPNWWKGACHGQ), identical to the related rat and mouse sequences.

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.

applications :

reactivity :

Human, Mouse, Rat

predicted reactivity :

Bovine

image labels :

Figure 1. Western blot analysis of GRB2 using anti-GRB2 antibody (PA1579). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. Lane 2: rat brain tissue lysates. Lane 3: rat spleen tissue lysates. Lane 4: mouse spleen tissue lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (Catalog # PA1579) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25KD. The expected band size for GRB2 is at 25KD.

background :

GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication. The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

competitor equivalent skus :

sc 137056 sc 13953 sc 28839 sc 137074 sc 17813 sc 8034 sc 365619 sc 255 sc 503 sc 255 G

research category :

Adapters, Cancer, Cytoplasmic, G Protein Signaling, Ras Family, Signal Transduction, Small G Proteins

synonyms :

Growth factor receptor-bound protein 2;Adapter protein GRB2;Protein Ash;SH2/SH3 adapter GRB2;GRB2;ASH;

gene full name :

Growth factor receptor-bound protein 2

molecular weight :

25206 MW

protein function :

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

subcellular localization :

Nucleus . Cytoplasm . Endosome . Golgi apparatus .

protein name :

Growth factor receptor-bound protein 2

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

sequence similarities :

Belongs to the GRB2/sem-5/DRK family.

last modified :

5/5/19 1:38