Anti-Fas Ligand/FASLG Antibody | Boster PA1576 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Fas Ligand/FASLG Antibody

catalog :

PA1576

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

product information

sku :

PA1576

status :

Enabled

name :

Anti-Fas Ligand/FASLG Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

FASLG

price various sizes :

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for Fas Ligand/FASLG detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. Fas Ligand/FASLG information: Molecular Weight: 31485 MW; Subcellular Localization: Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane.

short description :

Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Human.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

P48023

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(263-281aa ELSLVNFEESQTFFGLYKL).

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Western blot, 0.1-0.5ug/ml, Human.

applications :

IHC, WB

reactivity :

Human

predicted reactivity :

Monkey

image labels :

Anti-FASLG antibody, PA1576, Western blotting. All lanes: Anti FASLG (PA1576) at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: HL60 Whole Cell Lysate at 40ug. Predicted bind size: 31 KD. Observed bind size: 31 KD

background :

Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

competitor equivalent skus :

sc 18897 sc 19681 sc 33716 sc 6237 sc 71096 sc 834 sc 834 G sc 956 sc 956 G sc 957

research category :

Apoptosis, Caspases Etc, Cell Biology, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Intracellular, Lymphoid, Receptors, Stem Cells, T Lymphocytic Lineage, Tnf Superfamily

synonyms :

Tumor necrosis factor ligand superfamily member 6;Apoptosis antigen ligand;APTL;CD95 ligand;CD95-L;Fas antigen ligand;Fas ligand;FasL;CD178;Tumor necrosis factor ligand superfamily member 6, membrane form;Tumor necrosis factor ligand superfamily member 6, soluble form;Receptor-binding FasL ectodomain;Soluble Fas ligand;sFasL;ADAM10-processed FasL form;APL;FasL intracellular domain;FasL ICD;SPPL2A-processed FasL form;SPA;FASLG;APT1LG1, CD95L, FASL, TNFSF6;

gene full name :

Tumor necrosis factor ligand superfamily member 6

molecular weight :

31485 MW

protein function :

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

subcellular localization :

Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane.

protein name :

Tumor necrosis factor ligand superfamily member 6

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

sequence similarities :

Belongs to the tumor necrosis factor family.

last modified :

1/28/19 19:39