Anti-Aquaporin 3/AQP3 Antibody | Boster PA1488 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Aquaporin 3/AQP3 Antibody

catalog :

PA1488

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Boster product webpage

image

image 1 :

Figure 1. Western blot analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Mouse Kidney Tissue Lysate. Lane 4: MM453 Whole Cell Lysate. Lane 5: SMMC-7721 Whole Cell Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 3 antigen affinity purified polyclonal antibody (Catalog # PA1488) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 3 at approximately 32KD. The expected band size for Aquaporin 3 is at 32KD.

image 2 :

Figure 2. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

image 3 :

Figure 3. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in immunocytochemical section of HELA cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

product information

SKU :

PA1488

Product Name :

Anti-Aquaporin 3/AQP3 Antibody

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Monkey, Mouse, Rat

Predicted Reactivity :

Bovine

Application(s) :

Flow Cytometry, IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat, MonkeyImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human.Flow Cytometry, 1-3μg/1x10^6 cells, Human

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Aquaporin 3/AQP3 Antibody catalog # PA1488. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

AQP3

Uniprot ID :

Q92482

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3 (278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Aquaporin-3

Synonyms :

Aquaporin-3; AQP-3; Aquaglyceroporin-3; AQP3;

Protein Name :

Aquaporin-3

Molecular Weight :

31544 MW

Protein Function :

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).

Subcellular Localization :

Basolateral cell membrane; Multi-pass membrane protein. In collecting ducts of kidney.

Tissue Specificity :

Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Sequence Similarities :

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Background :

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Research Category :

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

more info or order :

Boster product webpage