Anti-Lipoamide Dehydrogenase/DLD Antibody | Boster PA1463 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Lipoamide Dehydrogenase/DLD Antibody

catalog :

PA1463

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

SKU :

PA1463

Product Name :

Anti-Lipoamide Dehydrogenase/DLD Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

IF, IHC-P, IHC-F, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Rat, Mouse. Immunocytochemistry , 0.5-1µg/ml, Human. Immunocytochemistry/Immunofluorescence, 5µg/ml, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

DLD

Uniprot ID :

P09622

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human DLD (492-509aa EAFREANLAASFGKSINF), different from the related mouse and rat sequences by one amino acid.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Dihydrolipoyl dehydrogenase, mitochondrial

Synonyms :

Dihydrolipoyl dehydrogenase, mitochondrial; 1.8.1.4; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; DLD; GCSL, LAD, PHE3;

Protein Name :

Dihydrolipoyl dehydrogenase, mitochondrial

Molecular Weight :

54177 MW

Protein Function :

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Subcellular Localization :

Mitochondrion matrix.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Background :

DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Research Category :

Cell Biology, Cell Cycle, Cell Differentiation, Developmental Biology, Embryogenesis, Lineage Specification, Nervous System Development, Neurogenesis, Neurology Process, Neuroscience, Organogenesis