Anti-beta Defensin 1/DEFB1 Antibody | Boster PA1450 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-beta Defensin 1/DEFB1 Antibody

catalog :

PA1450

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

Boster product webpage

citations: 1

Reference
Karadag R, Bayram N, Oguztuzun S, Bayramlar H, Bozer B, Sımşek G, et al. An investigation of human beta-defensins and cathelicidin expression in patients with pterygium. Arq Bras Oftalmol. 2017;80:277-280 pubmed publisher

image

image 1 :

Anti-beta Defensin 1 antibody, PA1450, IHC(P). IHC(P): Lung Cancer Tissue

image 2 :

Anti-beta Defensin 1 antibody, PA1450,Western blotting. All lanes: Anti Defensin 1 (PA1450) at 0.5ug/ml. WB : COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 7KD. Observed bind size: 7KD

product information

SKU :

PA1450

Product Name :

Anti-beta Defensin 1/DEFB1 Antibody

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-beta Defensin 1/DEFB1 Antibody catalog # PA1450. Tested in IHC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

DEFB1

Uniprot ID :

P60022

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human beta Defensin 1 (52-68aa FTKIQGTCYRGKAKCCK).

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Beta-defensin 1

Synonyms :

Beta-defensin 1; BD-1; hBD-1; Defensin, beta 1; DEFB1; BD1, HBD1;

Protein Name :

Beta-defensin 1(BD-1/hBD-1)

Molecular Weight :

7420 MW

Protein Function :

Has bactericidal activity.

Subcellular Localization :

Secreted.

Tissue Specificity :

Plasma

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the beta-defensin family.

Background :

DEFB1,Beta-defensin 1, is a protein that in humans is encoded by the DEFB1 gene. DEFB1 belongs to the defensin family. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. The DEFB1 gene spans more than 7 kb and includes a large 6,962-bp intron. By 2-color fluorescence in situ hybridization on both metaphase chromosomes and released chromatin fiber, the DEFB1gene is mapped to 8p23.2-p23.1, within 100 to 150 kb of the gene for human neutrophil defensin-alpha-1. DEFB1may augment neonatal host defenses through antimicrobial effects or prime the adaptive immune system at mucosal surfaces.

more info or order :

Boster product webpage