Anti-PPAR alpha/PPARA Antibody | Boster PA1412 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-PPAR alpha/PPARA Antibody

catalog :

PA1412

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

sku :

PA1412

status :

Enabled

name :

Anti-PPAR alpha/PPARA Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

PPARA

price various sizes :

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for PPAR alpha/PPARA detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. PPAR alpha/PPARA information: Molecular Weight: 52347 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.

short description :

Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

P23204

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids.

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat . Immunocytochemistry , 0.5-1ug/ml, Human, Mouse, Rat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human, Mouse, Rat. Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat.

applications :

ICC, WB

reactivity :

Human, Mouse, Rat

predicted reactivity :

Hamster

image labels :

Anti-PPAR alpha antibody, PA1412, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Cardiac Muscle Tissue Lysate. Lane 4: MM231 Cell Lysate. Lane 5: HELA Cell Lysate. Lane 6: SMMC Cell Lysate. Lane 7: HT1080 Cell Lysate. Lane 8: SW620 Cell Lysate Anti-PPAR alpha antibody, PA1412, IHC(P). IHC(P): Human Mammary Cancer Tissue. Anti-PPAR alpha antibody, PA1412, IHC(P). IHC(P): Human Mammary Cancer Tissue. Anti-PPAR alpha antibody, PA1412, IHC(P). IHC(P): Mouse Intestine Tissue

background :

Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

competitor equivalent skus :

sc 130640 sc 1982 sc 1982 X sc 1985 sc 1985 X sc 398394 sc 398394 X sc 9000 sc 9000 X

research category :

Atherosclerosis, Cancer, Cardiovascular, Co-Activators/Co-Repressors, Diabetes, Diabetes Associated, Epigenetics And Nuclear Signaling, Fatty Acid Oxidation, Fatty Acids, Heart Disease, Lipid And Lipoprotein Metabolism, Lipids / Lipoproteins, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Nuclear Receptors, Nuclear Signaling Pathways, Nucleotide Metabolism, Obesity, Pathways And Processes, Redox Metabolism, Transcription

synonyms :

Peroxisome proliferator-activated receptor alpha;PPAR-alpha;Nuclear receptor subfamily 1 group C member 1;Ppara;Nr1c1, Ppar;

gene full name :

Peroxisome proliferator-activated receptor alpha

molecular weight :

52347 MW

protein function :

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

subcellular localization :

Nucleus .

tissue specificity :

Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.

protein name :

Peroxisome proliferator-activated receptor alpha(PPAR-alpha)

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

sequence similarities :

Belongs to the nuclear hormone receptor family. NR1 subfamily.

last modified :

1/28/19 19:39