Anti-CD105/ENG Antibody | Boster PA1395 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CD105/ENG Antibody

catalog :

PA1395

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

product information

sku :

PA1395

status :

Enabled

name :

Anti-CD105/ENG Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

ENG

price various sizes :

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for CD105/ENG detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. CD105/ENG information: Molecular Weight: 70578 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Endoglin is restricted to endothelial cells in all tissues except bone marrow.

short description :

Rabbit IgG polyclonal antibody for Endoglin(ENG) detection. Tested with WB, IHC-P in Human.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

P17813

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human CD105(26-44aa ETVHCDLQPVGPERGEVTY).

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Western blot, 0.1-0.5ug/ml, Human.

applications :

IHC, WB

reactivity :

Human

image labels :

Figure 1. Western blot analysis of CD105 using anti-CD105 antibody (PA1395). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD105 antigen affinity purified polyclonal antibody (Catalog # PA1395) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD105 at approximately 95KD. The expected band size for CD105 is at 71KD. Anti-CD105 antibody, PA1395, IHC(P). IHC(P): Human Mammary Cancer Tissue

background :

Endoglin(Osler-Rendu-Weber syndrome 1), CD105, is a type I membrane glycoprotein located on cell surfaces and is a part of the TGF beta receptor complex. Its gene is mapped to human chromosome 8. The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts and smooth muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling and has been found to be elevated in pregnant women who subsequently develop preeclampsia.

competitor equivalent skus :

sc 18838 sc 19790 sc 20072 sc 20632 sc 21787 sc 23838 sc 376381 sc 53999 sc 57099 sc 6199

research category :

Epigenetics And Nuclear Signaling, Neurogenesis, Neurology Process, Neuroscience, Transcription

synonyms :

Endoglin;CD105;ENG;END;

gene full name :

Endoglin

molecular weight :

70578 MW

protein function :

Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3.

subcellular localization :

Membrane; Single-pass type I membrane protein.

tissue specificity :

Endoglin is restricted to endothelial cells in all tissues except bone marrow.

protein name :

Endoglin

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

last modified :

5/7/19 7:59