Anti-CD34 Antibody | Boster PA1334 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CD34 Antibody

catalog :

PA1334

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; fig 5i	Park M, Kim A, Manandhar S, Oh S, Jang G, Kang L, et al. CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity. elife. 2019;8: pubmed publisher
	Ayme Dietrich E, Lawson R, Cote F, de Tapia C, Da Silva S, Ebel C, et al. The role of 5-HT2B receptors in mitral valvulopathy: bone marrow mobilization of endothelial progenitors. Br J Pharmacol. 2017;174:4123-4139 pubmed publisher

image

image 1 :

Anti-CD34 antibody, PA1334, Western blotting. Lane 1: Recombinant Human CD34 Protein 10ng. Lane 2: Recombinant Human CD34 Protein 5ng. Lane 3: Recombinant Human CD34 Protein 2.5ng

image 2 :

Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Rat Kidney Tissue

image 3 :

Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Human Lung Cancer Tissue

product information

SKU :

PA1334

Product Name :

Anti-CD34 Antibody

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

Flow Cytometry, IHC-P, IHC-F, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Mouse, Rat, Human. Immunocytochemistry, 0.5-1µg/ml, Human. Flow Cytometry, 1-3µg/1x10^6 cells, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CD34 Antibody catalog # PA1334. Tested in Flow Cytometry, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

CD34

Uniprot ID :

P28906

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human CD34 (366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Hematopoietic progenitor cell antigen CD34

Synonyms :

Hematopoietic progenitor cell antigen CD34; CD34; CD34;

Protein Name :

Hematopoietic progenitor cell antigen CD34

Molecular Weight :

40716 MW

Protein Function :

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Belongs to the CD34 family.

Background :

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Research Category :

Cancer, Cardiogenesis, Cardiovascular, Cell Type Marker, Cell Type Markers, Developmental Biology, Hematopoietic Progenitors, Immunology, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Neuron Marker, Neuroscience, Stem Cells, Surface Molecules, Tumor Biomarkers

more info or order :

Boster product webpage