antibody

Anti-TrkA/NTRK1 Antibody

PA1321

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Enabled

Anti-TrkA/NTRK1 Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

NTRK1

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for TrkA/NTRK1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. TrkA/NTRK1 information: Molecular Weight: 87497 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity); Tissue Specificity: Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.

Rabbit IgG polyclonal antibody for High affinity nerve growth factor receptor(NTRK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

P04629

Rabbit

A synthetic peptide corresponding to a sequence in the middle region of human TrkA(342-361aa RHGCLRLNQPTHVNNGNYTL), identical to the related rat and mouse sequences.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat.

IHC, WB

Human, Mouse, Rat

Hamster

Anti-TrkA antibody, PA1321, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Mouse Brain Tissue Lysate. Lane 3: U87 Cell Lysate. Lane 4: SHG Cell Lysate. Lane 5: NEURO Cell Lysate. Anti-TrkA antibody, PA1321, IHC(P). IHC(P): Rat Brain Tissue

Trk A(Neurotrophic tyrosine kinase receptor A) is the high affinity catalytic receptor for the neurotrophin, Nerve Growth Factor(NGF). Higher affinity binding of NGFR can achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA(NTRK1), TRKB(NTRK2), and TRKC(NTRK3). TRKA, TRKB, and TRKC are specific for or "preferred by" NGF, NTF4(Neurotrophin-4) and BDNF, and NTF3(Neurotrophin-3), respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity. The absence of TrkA(NGFR) expression was associated with a strong increase in the Sp3 repressor short isoform(s) and a lack of the Sp3 activator long isoform. Sp3 is a bifunctional transcription factor that has been reported to stimulate or repress the transcription of numerous genes. Indo et al.(1996) concluded that defects in TRKA cause CIPA(Congenital insensitivity to pain with anhidrosis) and that the NGF-TRKA system has a crucial role in the development and function of the nociceptive reception as well as establishment of thermoregulation via sweating in humans. These results also implicate genes encoding other TRK and neurotrophin family members as candidates for developmental defect(s) of the nervous system.

sc 28735 sc 10215 sc 10217 sc 26950 sc 26952

Cancer, Growth And Development, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors

High affinity nerve growth factor receptor;2.7.10.1;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;Trk-A;gp140trk;p140-TrkA;NTRK1;MTC, TRK, TRKA;

High affinity nerve growth factor receptor

87497 MW

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras- PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.

Cell membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).

Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.

High affinity nerve growth factor receptor

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

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