antibody

Anti-LXR alpha/NR1H3 Antibody

PA1246

100μg/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

Anti-LXR alpha/NR1H3 Antibody

100μg/vial

Polyclonal

Rabbit

Rat, Mouse, Human

Hamster

WB

Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-LXR alpha/NR1H3 Antibody catalog # PA1246. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

NR1H3

Q13133

A synthetic peptide corresponding to a sequence at the C-terminus of human LXR alpha (375-398aa QVERLQHTYVEALHAYVSINHPHD), identical to the related rat and mouse sequences.

Lyophilized

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Oxysterols receptor LXR-alpha

Oxysterols receptor LXR-alpha; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3; NR1H3; LXRA;

Oxysterols receptor LXR-alpha

50396 MW

Nuclear receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Exhibits a ligand-dependent transcriptional activation activity (PubMed:25661920).

Nucleus.

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Belongs to the nuclear hormone receptor family. NR1 subfamily.

LXRA is a tissue-specific cofactor that permits RXRA to function as a potent 9cRA receptor with a distinct target gene specificity. It specifically interacts with RXRA in vivo to form a functional heterodimer in which RXRA is the ligand-binding subunit. Additionally, LXR activity is critical for physiologic lipid metabolism and transport. LXRs are endogenous inhibitors of atherogenesis and are targets for therapeutic intervention in cardiovascular disease. Furthermore, LXRs and their ligands are negative regulators of macrophage inflammatory gene expression. LXR is also found that as a transcriptional switch that integrates hepatic glucose metabolism and fatty acid synthesis. The LXR-IDOL-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake.

Cancer, Cancer Metabolism, Cardiovascular, ChIP’ing Antibodies, Cholesterol Metabolism, Co-Factors, Cofactors, Vitamins / Minerals, Epigenetics and Nuclear Signaling, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Lipids / Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Lipids And Lipoproteins, Nuclear Receptors, Nuclear Signaling Pathways, Pathways And Processes, Signal Transduction, Signaling Pathway, Transcription