antibody

Anti-ROCK2 Antibody

PA1242

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Enabled

Anti-ROCK2 Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

ROCK2

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for ROCK2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. ROCK2 information: Molecular Weight: 160900 MW; Subcellular Localization: Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

O75116

Rabbit

A synthetic peptide corresponding to a sequence at the N-terminus of human ROCK2(35-51aa RDPRSPINVESLLDGLN), identical to the related rat and mouse sequences.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.

IHC, WB

Human, Mouse, Rat

Chicken

Anti-ROCK2 antibody, PA1242, Western blotting. Lane 1: MCF-7 Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: MM453 Cell Lysate Anti-ROCK2 antibody, PA1242, IHC(P). IHC(P): Human Rectal Cancer Tissue Figure 3. Western blot analysis of ROCK2 using anti-ROCK2 antibody (PA1242). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # PA1242) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161KD. The expected band size for ROCK2 is at 161KD.

Rho-associated kinase(ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al.(2003) concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

sc 100425 sc 1851 sc 1852 sc 365275 sc 398519 sc 5561

Apoptosis, Cancer, Cell Biology, Intracellular, Kinases, Protein Phosphorylation, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction

Rho-associated protein kinase 2;2.7.11.1;Rho kinase 2;Rho-associated, coiled-coil-containing protein kinase 2;Rho-associated, coiled-coil-containing protein kinase II;ROCK-II;p164 ROCK-2;ROCK2;KIAA0619;

Rho-associated protein kinase 2

160900 MW

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Rho-associated protein kinase 2

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

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