Anti-Dopamine Receptor D1/DRD1 Antibody | Boster PA1231 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Dopamine Receptor D1/DRD1 Antibody

catalog :

PA1231

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

sku :

PA1231

status :

Enabled

name :

Anti-Dopamine Receptor D1/DRD1 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

DRD1

price various sizes :

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for DOPAMINE RECEPTOR D1/DRD1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. DOPAMINE RECEPTOR D1/DRD1 information: Molecular Weight: 49293 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14; Tissue Specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle.

short description :

Rabbit IgG polyclonal antibody for D(1A) dopamine receptor(DRD1) detection. Tested with WB in Human;Mouse;Rat.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

P21728

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human DRD1 (425-437aa DYDTDVSLEKIQP), identical to the related rat and mouse sequences.

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.

applications :

reactivity :

Human, Mouse, Rat

predicted reactivity :

Canine, Horse, Monkey, Rabbit

image labels :

Figure 1. Western blot analysis of DRD1/Dopamine Receptor D1 using anti-DRD1/Dopamine Receptor D1 antibody (PA1231). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates (positive control), . Lane 2: rat brain tissue lysates (positive control), . Lane 3: rat testis tissue lysates (negative control), . Lane 4: rat ovary tissue lysates (negative control). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD1/Dopamine Receptor D1 antigen affinity purified polyclonal antibody (Catalog # PA1231) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRD1/Dopamine Receptor D1 at approximately 72KD. The expected band size for DRD1/Dopamine Receptor D1 is at 49KD.

background :

Dopamine receptor D1, also known as DRD1, is a human gene. It is the most highly expressed DA receptor subtype among the DA receptor family. Receptors for dopamine have been classified into two functional types, D1 and D2. They belong to the family of receptors acting through G(or guanine nucleotide-binding) proteins. D2 receptors inhibit adenylyl cyclase, but D1 receptors stimulate adenylyl cyclase and activate cyclic AMP-dependent protein kinases. Dopamine D1 and D2 receptors are targets of drug therapy in many psychomotor disorders, including Parkinson's disease and schizophrenia, and may also have a role in drug addiction and alcoholism. D1 receptors regulate neuron growth and differentiation, influence behaviour and modify dopamine D2 receptor-mediated events. And the presence of a D1 receptor gene restriction fragment length polymorphism will be helpful for future disease linkage studies. DRD1 also regulates the neurochemical architecture of the striatum and is critical for the normal expression of motor activity.

competitor equivalent skus :

sc 104093 sc 292156 sc 104091 sc 376185

research category :

Neuroscience, Neurotransmission, Receptors / Channels

synonyms :

D(1A) dopamine receptor;Dopamine D1 receptor;DRD1;

gene full name :

D(1A) dopamine receptor

molecular weight :

49293 MW

protein function :

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

subcellular localization :

Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.

tissue specificity :

Detected in caudate, nucleus accumbens and in the olfactory tubercle.

protein name :

D(1A) dopamine receptor

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

last modified :

1/28/19 19:39