Anti-Aquaporin 5/AQP5 Antibody | Boster PA1230 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Aquaporin 5/AQP5 Antibody

catalog :

PA1230

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Boster product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 2c	Guo H, Kazadaeva Y, Ortega F, Manjunath N, Desai T. Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung. Dev Biol. 2017;430:214-223 pubmed publisher

image

image 1 :

Anti-Aquaporin 5 antibody, PA1230, Western blotting. Working concentration: 0.5ug/ml. Lane 1: Rat Lung Tissue Lysate. Lane 2: Rat Testis Tissue Lysate

image 2 :

Anti-Aquaporin 5 antibody, PA1230, IHC(P). IHC(P): Rat Lung Tissue Lysate. Working concentration: 1ug/ml

product information

SKU :

PA1230

Product Name :

Anti-Aquaporin 5/AQP5 Antibody

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Mouse, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Rat, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Aquaporin 5/AQP5 Antibody catalog # PA1230. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

AQP5

Uniprot ID :

Q9WTY4

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse AQP5 (246-265aa EEDWEDHREERKKTIELTAH), identical to the related rat sequence.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Aquaporin-5

Synonyms :

Aquaporin-5; AQP-5; Aqp5;

Protein Name :

Aquaporin-5(AQP-5)

Molecular Weight :

28274 MW

Protein Function :

Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.

Subcellular Localization :

Membrane; Multi-pass membrane protein.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins (AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0 (MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP4 in ciliary epithelium and retinal M ller cells, and AQP5 in corneal and lacrimal gland epithelia. Among the seven human aquaporins cloned to date (AQPs 0-6), genes encoding the four most closely related aquaporins (AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions.

Research Category :

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

more info or order :

Boster product webpage