Anti-Aquaporin 4/AQP4 Antibody | Boster PA1220 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Aquaporin 4/AQP4 Antibody

catalog :

PA1220

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

PA1220

Product Name :

Anti-Aquaporin 4/AQP4 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Rat, Mouse, Human

Predicted Reactivity :

Hamster

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Aquaporin 4/AQP4 Antibody catalog # PA1220. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

AQP4

Uniprot ID :

P55087

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (172-188aa FTIFASCDSKRTDVTGS), identical to the related rat and mouse sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Aquaporin-4

Synonyms :

Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4; AQP4;

Protein Name :

Aquaporin-4(AQP-4)

Molecular Weight :

34830 MW

Protein Function :

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

Subcellular Localization :

Membrane; Multi-pass membrane protein.

Tissue Specificity :

Brain - muscle >> heart, kidney, lung, and trachea.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Background :

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1.Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Research Category :

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction