Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody

catalog :

PA1203

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

PA1203

Product Name :

Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody catalog # PA1203. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

CHRNA1

Uniprot ID :

P02708

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1 (22-36aa EHETRLVAKLFKDYS), identical to the related rat sequence, different from the related mouse sequence by one amino acid.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Acetylcholine receptor subunit alpha

Synonyms :

Acetylcholine receptor subunit alpha; CHRNA1; ACHRA, CHNRA;

Protein Name :

Acetylcholine receptor subunit alpha

Molecular Weight :

54546 MW

Protein Function :

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subcellular Localization :

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue Specificity :

Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Background :

CHRNA, also termed ACHRA, is mapped on 2q24-q32.This gene encodes the alpha subunit of the muscle acetylcholine receptor, which is the main target of pathogenic autoantibodies in autoimmune myasthenia gravis. The protein-coding sequence of the human alpha subu gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule.

Research Category :

Auditory System, Calcium Channels, Calcium Signaling, Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Receptors / Channels, Sensory System, Signal Transduction, Signaling Pathway