Anti-Sonic Hedgehog/SHH Antibody | Boster PA1072-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Sonic Hedgehog/SHH Antibody

catalog :

PA1072-1

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

PA1072-1

Product Name :

Anti-Sonic Hedgehog/SHH Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Sonic Hedgehog/SHH Antibody catalog # PA1072-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

SHH

Uniprot ID :

Q15465

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog (203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Sonic hedgehog protein

Synonyms :

Sonic hedgehog protein; SHH; HHG-1; Sonic hedgehog protein N-product; Sonic hedgehog protein C-product; SHH;

Protein Name :

Sonic hedgehog protein

Molecular Weight :

49607 MW

Protein Function :

Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

Subcellular Localization :

Sonic hedgehog protein C-product: Secreted, extracellular space. The C-terminal peptide diffuses from the cell.

Tissue Specificity :

Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the hedgehog family.

Background :

The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.

Research Category :

Developmental Families, Domain Families, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Hypoxia, Intracellular, Metabolism, Metabolism Processes, Neural Stem Cells, Oncoproteins/Suppressors, Organogenesis, Pathways And Processes, Response To Hypoxia, Secreted, Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Tumor Suppressors