Anti-Nogo A/RTN4 Antibody | Boster PA1060 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Nogo A/RTN4 Antibody

catalog :

PA1060

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

sku :

PA1060

status :

Enabled

name :

Anti-Nogo A/RTN4 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

RTN4

price various sizes :

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for NOGO/RTN4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. NOGO/RTN4 information: Molecular Weight: 129931 MW; Subcellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains; Tissue Specificity: Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific.

short description :

Rabbit IgG polyclonal antibody for Reticulon-4(RTN4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

Q9NQC3

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Nogo A(1170-1192aa NKNVKDAMAKIQAKIPGLKRKAE), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Rat, Human, Mouse, By Heat. Western blot, 0.1-0.5ug/ml, Mouse, Rat, Human.

applications :

IHC, WB

reactivity :

Human, Mouse, Rat

predicted reactivity :

Chicken

image labels :

Anti-Nogo A antibody, PA1060, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Mouse Brain Tissue Lysate. Lane 4: Mouse Brain Tissue Lysate. Anti-Nogo A antibody, PA1060, IHC(P). IHC(P): Rat Brain Tissue

background :

Human neurite outgrowth inhibitor(NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.

competitor equivalent skus :

sc 366555 sc 5715 sc 5716 sc 5713

research category :

Neurology Process, Neuroscience

synonyms :

Reticulon-4;Foocen;Neurite outgrowth inhibitor;Nogo protein;Neuroendocrine-specific protein;NSP;Neuroendocrine-specific protein C homolog;RTN-x;Reticulon-5;RTN4;KIAA0886, NOGO;My043, SP1507;

gene full name :

Reticulon-4

molecular weight :

129931 MW

protein function :

Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.

subcellular localization :

Endoplasmic reticulum membrane; Multi-pass membrane protein. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.

tissue specificity :

Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific.

protein name :

Reticulon-4

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

sequence similarities :

Contains 1 reticulon domain.

last modified :

1/28/19 19:39