Anti-Hsp40/DNAJB1 Antibody | Boster PA1043 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Hsp40/DNAJB1 Antibody

catalog :

PA1043

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

PA1043

Product Name :

Anti-Hsp40/DNAJB1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Rat, Mouse, Human

Predicted Reactivity :

Hamster

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Hsp40/DNAJB1 Antibody catalog # PA1043. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

DNAJB1

Uniprot ID :

P25685

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp40 (317-332aa EFEVIFPERIPQTSRT), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

DNAJ homolog subfamily B member 1

Synonyms :

DNAJ homolog subfamily B member 1; DNAJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DNAJ protein 1; hDj-1; DNAJB1; DNAJ1, HDJ1, HSPF1;

Protein Name :

DNAJ homolog subfamily B member 1

Molecular Weight :

38044 MW

Protein Function :

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.

Subcellular Localization :

Cytoplasm. Nucleus. Nucleus, nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Contains 1 J domain.

Background :

The Hsp40 (heat shock protein with molecular size of approximately 40 kDa) is one of the mammalian homologues of bacterial DNAJ heat shock protein. Ohtsuka (1993) isolated a cDNA encoding a 40-kD heat-shock protein designated HSPF1. The deduced 340-amino acid HSPF1 protein is 34% identical to E. coli DNAJ and 34% and 36% identical to HSJ1 and HSJ2, respectively. HSPF1 gene spans over 7 kb and contains 3 exons and 2 introns. HSPF1 gene is mapped to chromosome 19p13.2.

Research Category :

Chaperones, Er Proteins, Heat Shock Proteins, Protein Trafficking, Signal Transduction