Anti-HIF-1-Alpha Antibody | Boster PA1041 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-HIF-1-Alpha Antibody

catalog :

PA1041

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

citations: 11

Reference
Wu H, Wang H, Zhang B, Zhang G, Zhang R, Zhang L. Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats. Neural Regen Res. 2012;7:421-5 pubmed publisher
Wang H, Wu H, Guo H, Zhang G, Zhang R, Zhan S. Increased hypoxia-inducible factor 1alpha expression in rat brain tissues in response to aging. Neural Regen Res. 2012;7:778-82 pubmed publisher
Wang J, Jing C, Shan K, Chen Y, Guo X, Cao Z, et al. Semaphorin 4D and hypoxia-inducible factor-1Î± overexpression is related to prognosis in colorectal carcinoma. World J Gastroenterol. 2015;21:2191-8 pubmed publisher
Wang T, Zhou Y, Chen X, Zhu A. Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1? expression. Braz J Med Biol Res. 2014;47:738-45 pubmed
Weng T, Xie Y, Yi L, Huang J, Luo F, Du X, et al. Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis. Osteoarthritis Cartilage. 2014;22:1197-205 pubmed publisher
Weng T, Xie Y, Huang J, Luo F, Yi L, He Q, et al. Inactivation of Vhl in osteochondral progenitor cells causes high bone mass phenotype and protects against age-related bone loss in adult mice. J Bone Miner Res. 2014;29:820-9 pubmed publisher
Wang F, Liu S, Xi S, Yan L, Wang H, Song Y, et al. Arsenic induces the expressions of angiogenesis-related factors through PI3K and MAPK pathways in SV-HUC-1 human uroepithelial cells. Toxicol Lett. 2013;222:303-11 pubmed publisher
Sun D, Wang Y, Liu C, Zhou X, Li X, Xiao A. Effects of nitric oxide on renal interstitial fibrosis in rats with unilateral ureteral obstruction. Life Sci. 2012;90:900-9 pubmed publisher
He C, Sun X, Qiao H, Jiang X, Wang D, Jin X, et al. Downregulating hypoxia-inducible factor-2? improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma. Cancer Sci. 2012;103:528-34 pubmed publisher
Mou K, Chen M, Mao Q, Wang P, Ni R, Xia X, et al. AQP-4 in peritumoral edematous tissue is correlated with the degree of glioma and with expression of VEGF and HIF-alpha. J Neurooncol. 2010;100:375-83 pubmed publisher
Wu W, Chen X, Hu C, Li J, Yu Z, Cai W. Transplantation of neural stem cells expressing hypoxia-inducible factor-1alpha (HIF-1alpha) improves behavioral recovery in a rat stroke model. J Clin Neurosci. 2010;17:92-5 pubmed publisher

product information

sku :

PA1041

name :

Anti-HIF-1-Alpha Antibody

price :

200 USD

immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human HIF-1-alpha(8-21aa NDKKKISSERRKEK), different from the related rat and mouse sequences by two amino acids.

form :

Lyophilized

host :

Rabbit

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

purification :

Immunogen affinity purified.

clonality :

Polyclonal

description :

Rabbit IgG polyclonal antibody for Hypoxia-inducible factor 1-alpha(HIF1A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

size :

100 ug/vial

reactivity :

Human; Mouse; Rat

applications :

IHC-P; WB

background :

HIF-1 alpha(Hypoxia-inducible factor 1 alpha,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1 alpha transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1 alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1 alpha regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

cross reactivity :

No cross reactivity with other proteins

gene full name :

Hypoxia-inducible factor 1-alpha

gene name :

HIF1A

predicted reactivity :

Chicken

protein function :

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

protein name :

Hypoxia-inducible factor 1-alpha(HIF-1-alpha/HIF1-alpha)

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

research category :

cell biology apoptosis intracellular bcl2 family cardiovascular hypoxia hif microbiology interspecies interaction host virus interaction epigenetics and nuclear signaling transcription domain families hlh / leucine zipper hlh cell biology mitochondrial cardiovascular/immune cancer invasion/microenvironment heart cancer metabolism response to hypoxia metabolism pathways and processes mitochondrial metabolism mitochondrial markers metabolism processes types of disease cell death

sequence similarities :

Contains 1 bHLH (basic helix-loop-helix) domain.

storage :

At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquotted and stored frozen at -20ËšC for a longer time.Avoid repeated freezing and thawing.

subcellular localization :

Cytoplasm. Nucleus. Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.

synonyms :

Hypoxia-inducible factor 1-alpha;HIF-1-alpha;HIF1-alpha;ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;bHLHe78;Member of PAS protein 1;PAS domain-containing protein 8;HIF1A;BHLHE78, MOP1, PASD8;

tissue specificity :

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

uniprot id :

Q16665