Anti-Annexin V/ANXA5 Antibody | Boster PA1008 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Annexin V/ANXA5 Antibody

catalog :

PA1008

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:200	Rickelt S, Hynes R. Antibodies and methods for immunohistochemistry of extracellular matrix proteins. Matrix Biol. 2018;71-72:10-27 pubmed publisher

image

image 1 :

Anti-Annexin V antibody, PA1008, Western blotting. All lanes: Anti Annexin V (PA1008) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug. Lane 3: Rat Ovary Tissue Lysate at 50ug. Lane 4: Rat Lung Tissue Lysate at 50ug. Lane 5: MCF-7 Whole Cell Lysate at 40ug. Lane 6: SMMC Whole Cell Lysate at 40ug. Lane 7: A549 Whole Cell Lysate at 40ug. Lane 8: JURKAT Whole Cell Lysate at 40ug. Lane 9: SGC Whole Cell Lysate at 40ug. Lane 10: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 36KD. Observed bind size: 36KD

image 2 :

Anti-Annexin V antibody, PA1008, IHC(P). IHC(P): Rat Liver Tissue

image 3 :

Anti-Annexin V antibody, PA1008, ICC. ICC: HCT116 Cell

product information

SKU :

PA1008

Product Name :

Anti-Annexin V/ANXA5 Antibody

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

IHC, ICC, WB

Application Details :

Immunocytochemistry , 0.5-1µg/ml, HumanImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Annexin V/ANXA5 Antibody catalog # PA1008. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

ANXA5

Uniprot ID :

P08758

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human Annexin V (88-102aa SRLYDAYELKHALKG), identical to the related rat and mouse sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Annexin A5

Synonyms :

Annexin A5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; PP4; Placental anticoagulant protein I; PAP-I; Thromboplastin inhibitor; Vascular anticoagulant-alpha; VAC-alpha; ANXA5; ANX5, ENX2, PP4;

Protein Name :

Annexin A5

Molecular Weight :

35937 MW

Protein Function :

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Sequence Similarities :

Belongs to the annexin family.

Background :

Annexin V also know as endonnexin II (ENX2),or placental protein 4 (PP4). Endonexin II is a member of the family of Ca (2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Research Category :

Cell Biology, Cell Death, Invasion/Microenvironment

more info or order :

Boster product webpage