Anti-Alkaline Phosphatase/ALPL Antibody | Boster PA1004 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Alkaline Phosphatase/ALPL Antibody

catalog :

PA1004

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Boster product webpage

citations: 1

Reference
Liu Z, Au M, He T, Yang B, Liu B, Zhang L, et al. Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study. Biomed Res Int. 2019;2019:2075968 pubmed publisher

image

image 1 :

Anti-Alkaline Phosphatase antibody, PA1004, Western blotting. All lanes: Anti ALPL (PA1004) at 0.5ug/ml. WB: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 57KD. Observed bind size: 57KD

image 2 :

Anti-Alkaline Phosphatase antibody, PA1004, IHC(P). IHC(P): Rat Small Intestine Tissue

product information

SKU :

PA1004

Product Name :

Anti-Alkaline Phosphatase/ALPL Antibody

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Canine, Monkey

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

ALPL

Uniprot ID :

P05186

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase (21-35aa,EKEKDPKYWRDQAQE), different from the related rat and mouse sequences by two amino acids.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Alkaline phosphatase, tissue-nonspecific isozyme

Synonyms :

Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; 3.1.3.1 ; Alkaline phosphatase liver/bone/kidney isozyme; ALPL;

Protein Name :

Alkaline phosphatase, tissue-nonspecific isozyme(AP-TNAP/TNSALP)

Molecular Weight :

57305 MW

Protein Function :

This isozyme may play a role in skeletal mineralization.

Subcellular Localization :

Cell membrane ; Lipid-anchor, GPI-anchor.

Background :

Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Research Category :

Cancer, Cell Biology, Cell Cycle, Kinases/Phosphatases, Tags & Cell Markers, Tumor Biomarkers

more info or order :

Boster product webpage