Anti-Phospho-alpha Tubulin (Y272) Rabbit Monoclonal Antibody

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospho-alpha Tubulin (Y272) Rabbit Monoclonal Antibody

catalog :

P08382

quantity :

100 ug/vial

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

BEF-20

reactivity :

human

application :

western blot, immunocytochemistry

product information

sku :

P08382

status :

Enabled

name :

Anti-Phospho-alpha Tubulin (Y272) Rabbit Monoclonal Antibody

category name :

Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

TUBA1B

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99

clonality :

Monoclonal

clone number :

BEF-20

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

description :

Monoclonal antibody for TUBA1B detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, ICC, WB. Reactive species: Human TUBA1B information: Molecular Weight: 50152 MW; Subcellular Localization: Cytoplasm, cytoskeleton.

short description :

Anti-Phospho-alpha Tubulin (Y272) Rabbit Monoclonal Antibody tested for IF, ICC, WB in Human

size :

100 ug/vial

sample size available :

30ul for $99, contact us for details

uniprot id :

P68363

host :

Rabbit

immunogen :

A synthesized peptide derived from human Phospho-alpha Tubulin (Y272)

form :

Liquid

purification :

Affinity-chromatography

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.

application details :

WB 1:500-1:2000 . ICC/IF 1:50-1:200 .

applications :

IF, WB

reactivity :

Human

image labels :

Western blot analysis on Jurkat cell lysates treated with pervanadate using alpha Tubulin (phospho Y272) Antibody (P08382). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBA1B monoclonal antibody (Catalog # P08382) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBA1B

research category :

Signal Transduction, Tubulin

synonyms :

Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain;TUBA1B;

gene full name :

Tubulin alpha-1B chain

molecular weight :

50152 MW

protein function :

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

subcellular localization :

Cytoplasm, cytoskeleton.

last modified :

4/22/19 1:33