Anti-Phospho-SAPK/JNK (Thr183) Antibody | Boster P02706 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospho-SAPK/JNK (Thr183) Antibody

catalog :

P02706

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

sku :

P02706

status :

Enabled

name :

Anti-Phospho-SAPK/JNK (Thr183) Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

MAPK9

price various sizes :

5ul sample size / $40 100ul / $299 100ul+Free HRP Secondary BA1054 / $299 100ul+Free Biotin Secondary BA1003 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

description :

Polyclonal antibody for JNK2/MAPK9 detection. Host: Rabbit.Size: 100ul. Tested applications: IHC. Reactive species: Human. JNK2/MAPK9 information: Molecular Weight: 48139 MW; Subcellular Localization: Cytoplasm . Nucleus .

short description :

Rabbit Polyclonal Antibody for Phospho-SAPK/JNK (Thr183) (MAPK9) detection. Tested with WB, IHC in Human, Mouse, Rat.

size :

100 ul

sample size available :

5ul, free with shipping charge

uniprot id :

P45984

host :

Rabbit

immunogen :

Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK.

form :

Liquid

purification :

Immunogen affinity purified

storage :

Store at -20 C for one year, at 4 C for one month. We suggest the antibody be aliquotted into small vials and stored frozen at -20 C upon receiving. Avoid repeated freezing and thawing.

application details :

WB, 1:500-1:1000. IHC, 1:50-1:100.

applications :

IHC, WB

reactivity :

Human, Mouse, Rat

image labels :

Western blot analysis of extracts from 293 cells using SAPK/JNK(Phospho-Thr183) antibody P02706(Lane 2) and the same antibody preincubated with blocking peptide(Lane1). Western blot analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) antibody P02706. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Phospho-Thr183) antibody P02706(left) or the same antibody preincubated with blocking peptide(right).

research category :

Cancer, Cell Biology, Immunology, Innate Immunity, Mapk Pathway, Metabolism, Oncoproteins, Oncoproteins/Suppressors, Oxidative Stress, Pathways And Processes, Protein Phosphorylation, Redox Metabolism, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Tlr Signaling, Tumor Biomarkers

synonyms :

Mitogen-activated protein kinase 9;MAP kinase 9;MAPK 9;2.7.11.24;JNK-55;Stress-activated protein kinase 1a;SAPK1a;Stress-activated protein kinase JNK2;c-Jun N-terminal kinase 2;MAPK9;JNK2, PRKM9, SAPK1A;

gene full name :

Mitogen-activated protein kinase 9

molecular weight :

48139 MW

protein function :

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).

subcellular localization :

Cytoplasm . Nucleus .

last modified :

2/6/19 22:57