Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody | Boster P02067-1 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody

catalog :

P02067-1

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

FCG-18

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

product information

SKU :

P02067-1

Product Name :

Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

FCG-18

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IP, WB

Application Details :

WB 1:5000-1:10000 .IP 1:20

Description :

Boster Bio Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody catalog # P02067-1. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

RPA2

Uniprot ID :

P15927

Immunogen :

A synthesized peptide derived from human Phospho-RPA2 (T21)

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Replication protein A 32 kDa subunit

Synonyms :

Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34; RPA2; REPA2, RPA32, RPA34;

Molecular Weight :

29247 MW

Protein Function :

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Subcellular Localization :

Nucleus. Nucleus, PML body. Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.

Research Category :

DNA / RNA, DNA Damage & Repair, DNA Damage Response, DNA Synthesis, Epigenetics and Nuclear Signaling