antibody

Anti-Phospho-AMPK alpha1 (Ser496)Antibody

P00994

100 ul

polyclonal

domestic rabbit

nonconjugated

western blot, immunohistochemistry, immunocytochemistry

Enabled

Anti-Phospho-AMPK alpha1 (Ser496)Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

PRKAA1

5ul sample size / $40 100ul / $299 100ul+Free HRP Secondary BA1054 / $299 100ul+Free Biotin Secondary BA1003 / $299

Polyclonal

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

No

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Polyclonal antibody for AMPK alpha 1/PRKAA1 detection. Host: Rabbit.Size: 100ul. Tested applications: IF. Reactive species: Human. AMPK alpha 1/PRKAA1 information: Molecular Weight: 64009 MW; Subcellular Localization: Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters.

Rabbit Polyclonal Antibody for Phospho-AMPK alpha1 (Ser496)Antibody (PRKAA1) detection. Tested with WB, IHC, IF in Human.

100 ul

5ul, free with shipping charge

Q13131

Rabbit

Peptide sequence around phosphorylation site of serine 496 (S-G- S(P)-V-S) derived from Human AMPK alpha1.

Liquid

Immunogen affinity purified

Store at -20 C for one year, at 4 C for one month. We suggest the antibody be aliquotted into small vials and stored frozen at -20 C upon receiving. Avoid repeated freezing and thawing.

WB, 1:500-1:1000. IHC, 1:50-1:100. Immunofluorescence, 1:100-1:200.

IF, IHC, WB

Human

Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPK alpha1 (Phospho-Ser496) antibody P00994. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPK alpha1 (Phospho-Ser496) antibody P00994 (left) or the same antibody preincubated with blocking peptide (right). Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPK alpha1 (Phospho-Ser496) antibody P00994. Immunofluorescence staining of methanol-fixed Hela cells using AMPK alpha1(Phospho-Ser496) antibody P00994.

Metabolism Processes, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways And Processes, Protein Phosphorylation, Redox Metabolism, Response To Hypoxia, Ser / Thr Kinases, Signal Transduction

5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;2.7.11.1 ;Acetyl-CoA carboxylase kinase;ACACA kinase;2.7.11.27 ;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;2.7.11.31 ;Tau-protein kinase PRKAA1;2.7.11.26;PRKAA1;AMPK1;

5'-AMP-activated protein kinase catalytic subunit alpha-1

64009 MW

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters.

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