Anti-Phospho-CREB S133 Antibody | Boster P00577 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospho-CREB S133 Antibody

catalog :

P00577

quantity :

100 uL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

sku :

P00577

status :

Enabled

name :

Anti-Phospho-CREB S133 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

CREB1

price various sizes :

100uL / $329 100uL+Free HRP Secondary BA1054 / $329 100uL+Free Biotin Secondary BA1003 / $329

clonality :

Polyclonal

concentration :

0.064 mg/mL by UV absorbance at 280 nm

conjugate :

description :

Polyclonal antibody for CREB/CREB1 detection. Host: Rabbit.Size: 100uL. Tested applications: IHC. Reactive species: Human. CREB/CREB1 information: Molecular Weight: 36688 MW; Subcellular Localization: Nucleus .

short description :

Anti-CREB phospho S133 Antibody validated for WB in Human.

size :

100 uL

sample size available :

25ug for $99, contact us for details

uniprot id :

P16220

host :

Rabbit

immunogen :

CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

form :

Liquid

purification :

Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification.

storage :

Store vial at -20Â° C prior to opening. Aliquot contents and freeze at -20Â° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4Â° C as an undiluted liquid. Dilute only prior to immediate use.

application details :

Western Blot, 1:500 - 1:2, 000, human, mouse, rat. Direct ELISA, 1:1, 000 - 1:6, 000, human, mouse, rat. Immunohistochemistry: 20ug/ml, human, mouse, rat.

applications :

IHC, WB

reactivity :

Human, Mouse, Rat

image labels :

CREB was detected in paraffin-embedded sections of tonsillar lymphocytes tissue using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 20 ug/mL. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA. Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, R. Screaton, The Salk Institute for Biological Studies. Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.

background :

CREB1, also known as cAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene. It is mapped to 2q33.3. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.

competitor equivalent skus :

sc 273 sc 34863 sc 204 sc 34862 sc 421 sc 56796 sc 374035 sc 271146 sc 135804 sc 135803 sc 101550 sc 56795 sc 56794 sc 204 X sc 273 X sc 34863 X sc 34862 X sc 421 X sc 374035 X sc 271146 X sc 135804 X sc 135803 X

research category :

Domain Families, Epigenetics And Nuclear Signaling, Hlh / Leucine Zipper, Transcription, Transcription Factors

synonyms :

Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1;CREB1;

gene full name :

Cyclic AMP-responsive element-binding protein 1

molecular weight :

36688 MW

protein function :

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

subcellular localization :

Nucleus .

last modified :

4/11/19 19:52