antibody

Anti-Phospho-SMAD3 S423/S425 Antibody

P00059

100 uL

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Enabled

Anti-Phospho-SMAD3 S423/S425 Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

SMAD3

25ug sample size / $99 100uL / $339 100uL+Free HRP Secondary BA1054 / $339 100uL+Free Biotin Secondary BA1003 / $339

Polyclonal

1.0 mg/mL by UV absorbance at 280 nm

No

Polyclonal antibody for SMAD3 detection. Host: Rabbit.Size: 100uL. Tested applications: IHC. Reactive species: Human. SMAD3 information: Molecular Weight: 48081 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.

Anti-SMAD3 phospho S423/phospho S425 Antibody validated for WB in Human.

100 uL

25ug for $99, contact us for details

P84022

Rabbit

Liquid

No cross reactivity with other proteins.

Western Blot, 1:2, 000 - 1:20, 000, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken. Flow Cytometry, User Optimized, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken. Direct ELISA, 1:15, 000 - 1:30, 000, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken. Immunohistochemistry: 1:500, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken.

IHC, WB

Human, Mouse, Rat

Bovine, Chicken, Xenopus Laevis, Xenopus Tropicalis, Zebrafish

SMAD3 was detected in paraffin-embedded sections of epidermal keratinocytes at 40X using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 2.5 ug/mL. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA. Western blot analysis of endogenous SMAD3 expression in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF for 1 hour. Phosphorylated SMAD3 was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059). Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD. Western blot analysis of SMAD3 expression in HEK293 lysate (lane 1), HeLa Lysate (lane 2), MCF-7 Lysate (lane 3), Jurkat Lysate (lane 4), A431 Lysate (lane 5), A549 Lysate (lane 6), LNCap Lysate (lane 7), MOLT-4 Lysate (lane 8), Ramos Lysate (lane 9), Raji Lsyate (lane 10), A-172 Lysate (lane 11) and NIH/3T3 Lysate (lane 12). Phosphorylated SMAD3 at 35KD was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 1ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Mothers against decapentaplegic homolog 3 also known as SMAD family member 3 or SMAD3 is a protein that in humans is encoded by the SMAD3 gene. SMAD3 is a member of the SMAD family of proteins. It is located in 15q22.33. And it mediates the signals from the transforming growth factor beta (TGF-??) super family ligands that regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.

sc 101154

Cancer, Cancer Metabolism, Cytoplasmic, Epigenetics And Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Pathways And Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, Tgf Beta

Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3;SMAD3;MADH3;

Mothers against decapentaplegic homolog 3

48081 MW

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.

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