Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody

catalog :

P00059-1

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AHO-19

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

P00059-1

Product Name :

Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

AHO-19

Host :

Rabbit

Reactivity :

Human, Mouse

Application(s) :

IF, IHC, ICC, WB

Application Details :

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200

Description :

Boster Bio Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody catalog # P00059-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

SMAD3

Uniprot ID :

P84022

Immunogen :

A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Mothers against decapentaplegic homolog 3

Synonyms :

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3;

Molecular Weight :

48081 MW

Protein Function :

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Subcellular Localization :

Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.

Research Category :

Cancer Metabolism, Cytoplasmic, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Pathways And Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, Tgf Beta