Anti-Phospho-MDM2 S185 Antibody | Boster P00054 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospho-MDM2 S185 Antibody

catalog :

P00054

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunoprecipitation

product information

sku :

P00054

status :

Enabled

name :

Anti-Phospho-MDM2 S185 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

MDM2

price various sizes :

100ug / $329 100ug+Free HRP Secondary BA1054 / $329 100ug+Free Biotin Secondary BA1003 / $329

clonality :

Polyclonal

concentration :

1.0 mg/mL by UV absorbance at 280 nm

conjugate :

contents :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

description :

Anti-MDM2 phospho S185 Antibody validated for WB, IP, ELISA in Human;Mouse;Rat.

short description :

Anti-MDM2 phospho S185 Antibody validated for WB, IP, ELISA in Human;Mouse;Rat.

size :

100 ug

sample size available :

25ug for $99, contact us for details

uniprot id :

Q00987

host :

Rabbit

immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 177-198 of mouse MDM2.

form :

Liquid

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

application details :

Western Blot, 1:500 - 1:2, 000, human, mouse, rat. Direct ELISA, 1:3, 000 - 1:12, 000, human, mouse, rat. ImmunoPrecipitation: 1:100, human, mouse, rat.

applications :

ELISA, IP, WB

reactivity :

Human, Mouse, Rat

image labels :

Western blot analysis of MDM2 expression in untreated 293T whole cell lysate (lane 1) and 293T cell treated with IGF-1 (100 ng/ml) for 20 min prior to harvest (lane 2). MDM2 at 102KD was detected using rabbit anti-MDM2 pS185 Antigen Affinity purified polyclonal antibody (Catalog # P00054). The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

background :

Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

competitor equivalent skus :

sc 56154 sc 56430 sc 965 sc 813 sc 7918 sc 5304 sc 812 sc 13161 sc 965 X

research category :

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics And Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, P53 Pathway, Transcription, Ubiquitin & Ubiquitin Like Modifiers

synonyms :

E3 ubiquitin-protein ligase Mdm2;6.3.2.-;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2;MDM2;

gene full name :

E3 ubiquitin-protein ligase Mdm2

molecular weight :

55233 MW

protein function :

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation.

subcellular localization :

Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.

tissue specificity :

Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

last modified :

1/28/19 19:39