Anti-Phospho-ERK/MAPK (Thr202, Tyr204) Antibody | Boster P00030 product information

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product summary

company name :

Boster

product type :

other

product name :

Anti-Phospho-ERK/MAPK (Thr202, Tyr204) Antibody

catalog :

P00030

quantity :

100 ul

product information

sku :

P00030

status :

Enabled

name :

Anti-Phospho-ERK/MAPK (Thr202, Tyr204) Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

MAPK1

price various sizes :

100ul / $300 100ul+Free HRP Secondary BA1054 / $300 100ul+Free Biotin Secondary BA1003 / $300

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

description :

Polyconal antibody for ERK2/MAPK1 detection. Host: Rabbit.Size: 100ul. Tested applications: IHC. Reactive species: Human. ERK2/MAPK1 information: Molecular Weight: 41390 MW; Subcellular Localization: Cytoplasm, cytoskeleton, spindle . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Associated with the spindle during prometaphase and metaphase (By similarity). PEA15- binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization.

short description :

Phospho-specific Rabbit Anti-ERK/MAPK (Thr 202 , Tyr 204 ) Antibody validated for WB, IHC in human, mouse, rat.

size :

100 ul

uniprot id :

P28482

host :

Rabbit

immunogen :

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.

form :

Liquid

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

application details :

WB, 1:1, 000. IHC, 1:500.

applications :

IHC, WB

reactivity :

Human, Mouse, Rat

predicted reactivity :

Bovine, Canine, Chicken, Mouse, Xenopus

image labels :

Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr 202 /Tyr 204 (red) and nuclei (blue). Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr 202 /Tyr 204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).

research category :

Mapk Pathway, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction

synonyms :

Mitogen-activated protein kinase 1;MAP kinase 1;MAPK 1;2.7.11.24;ERT1;Extracellular signal-regulated kinase 2;ERK-2;MAP kinase isoform p42;p42-MAPK;Mitogen-activated protein kinase 2;MAP kinase 2;MAPK 2;MAPK1;ERK2, PRKM1, PRKM2;

gene full name :

Mitogen-activated protein kinase 1

molecular weight :

41390 MW

protein function :

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation.

subcellular localization :

Cytoplasm, cytoskeleton, spindle . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Associated with the spindle during prometaphase and metaphase (By similarity). PEA15- binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization.

protein name :

Mitogen-activated protein kinase 1

last modified :

2/6/19 22:57