Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody | Boster P00003 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody

catalog :

P00003

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

IFF-13

reactivity :

human, mouse

application :

western blot, immunohistochemistry

product information

SKU :

P00003

Product Name :

Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

IFF-13

Host :

Rabbit

Reactivity :

Human, Mouse, Pig

Application(s) :

IHC, WB

Application Details :

WB 1:500~1:1000 .IHC 1:50~1:100

Description :

Boster Bio Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody catalog # P00003. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Pig.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

MTOR

Uniprot ID :

P42345

Immunogen :

A synthesized peptide derived from human Phospho-mTOR (S2448)

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Serine/threonine-protein kinase mTOR

Synonyms :

Serine/threonine-protein kinase mTOR; 2.7.11.1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP, FRAP1, FRAP2, RAFT1, RAPT1;

Molecular Weight :

288892 MW

Protein Function :

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1- mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1- pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser- 758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422'. Regulates osteoclastogensis by adjusting the expression of CEBPB isoforms (By similarity).

Subcellular Localization :

Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side. Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side. Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side. Lysosome. Cytoplasm. Nucleus, PML body. Microsome membrane. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137).

Tissue Specificity :

Expressed in numerous tissues, with highest levels in testis.

Research Category :

Cancer, Cardiogenesis, Cardiovascular, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, DNA / RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Metabolism, Obesity, Transcription Factors/Regulators