Anti-Fibronectin Fn1 Monoclonal Antibody | Boster MA1116 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Fibronectin Fn1 Monoclonal Antibody

catalog :

MA1116

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

FN-15

The same clone is also sold as:

MilliporeSigma: SAB4200760, F7387

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1116

Product Name :

Anti-Fibronectin Fn1 Monoclonal Antibody

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

FN-15

Host :

Mouse

Reactivity :

Chicken, Human, Mouse

Application(s) :

IHC, ICC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, chicken, mouse, By Heat. Immunocytochemistry, 1µg/ml, Human, chicken, mouseImmunohistochemistry (Frozen Section), 1-2µg/ml, Human, chicken, mouse Western blot, 1-2µg/ml, Human, chicken, mouse.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Fibronectin Fn1 Monoclonal Antibody catalog # MA1116. Tested in IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

FN1

Uniprot ID :

P04937

Immunogen :

Fibronectin from human plasma.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Fibronectin

Synonyms :

Fibronectin; FN; Anastellin; Fn1;

Protein Name :

Fibronectin

Molecular Weight :

272511 MW

Protein Function :

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity).

Subcellular Localization :

Secreted, extracellular space, extracellular matrix.

Tissue Specificity :

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Contains 12 fibronectin type-I domains.

Background :

Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.