Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) | Boster MA1115 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)

catalog :

MA1115

quantity :

100μg/vial

price :

315 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AC-15

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Boster product webpage

citations: 34

Reference
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Zhao M, Chen Y, Dong X, Zhou J, Chen X, Wang H, et al. Folic acid protects against lipopolysaccharide-induced preterm delivery and intrauterine growth restriction through its anti-inflammatory effect in mice. PLoS ONE. 2013;8:e82713 pubmed publisher
Zhao M, Chen Y, Chen X, Dong X, Zhou J, Wang H, et al. Folic acid supplementation during pregnancy protects against lipopolysaccharide-induced neural tube defects in mice. Toxicol Lett. 2014;224:201-8 pubmed publisher
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Gao K, Li X, Zhang L, Bai L, Dong W, Gao K, et al. Transgenic expression of IL-33 activates CD8(+) T cells and NK cells and inhibits tumor growth and metastasis in mice. Cancer Lett. 2013;335:463-71 pubmed publisher
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image

image 1 :

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting. Lane 1: Rat Ovary Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: Rat Cardiac Muscle Tissue Lysate. Lane 4: Rat Brain Tissue Lysate. Lane 5: A453 Cell Lysate. Lane 6: HE

image 2 :

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Spleen Tissue Lysate. Lane 3: Rat Brain Tissue Lysate. Lane 4: Rat Kidney Tissue Lysate. Lane 5: HELA Cell Lysate. Lane 6: SMMC Cell

image 3 :

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P). IHC(P): Mouse Intestine Tissue

product information

SKU :

MA1115

Product Name :

Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)

Price :

315 USD

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

AC-15

Host :

Mouse

Reactivity :

Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat

Predicted Reactivity :

Bovine, Chicken, Canine, Hamster, Horse, Monkey, Rabbit, Zebrafish

Application(s) :

IHC, WB

Application Details :

Western blot, 0.05-0.1µg/ml, Human, mouse, rat, pig, monkey, rabbit, chicken. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, mouse, rat, By Heat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) catalog # MA1115. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

ACTB

Uniprot ID :

P60711

Immunogen :

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Actin, cytoplasmic 1

Synonyms :

Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; Actb;

Protein Name :

Actin, cytoplasmic 1

Molecular Weight :

41737 MW

Protein Function :

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular Localization :

Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Sequence Similarities :

Belongs to the actin family.

Background :

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Research Category :

Actin, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction

more info or order :

Boster product webpage