Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4)

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4)

catalog :

MA1106

quantity :

100μg/vial

price :

315 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1A4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Boster product webpage

citations: 23

Reference
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Li Q, Zhu Y, Zhang M, Chen L, Zhang Z, Du Y, et al. Chlorogenic acid inhibits hypoxia-induced pulmonary artery smooth muscle cells proliferation via c-Src and Shc/Grb2/ERK2 signaling pathway. Eur J Pharmacol. 2015;751:81-8 pubmed publisher
Qiu X, Fu K, Zhao X, Zhang Y, Yuan Y, Zhang S, et al. Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice. J Transl Med. 2015;13:28 pubmed publisher
Liu Z, Xu S, Huang X, Wang J, Gao S, Li H, et al. Cryptotanshinone, an orally bioactive herbal compound from Danshen, attenuates atherosclerosis in apolipoprotein E-deficient mice: role of lectin-like oxidized LDL receptor-1 (LOX-1). Br J Pharmacol. 2015;172:5661-75 pubmed publisher
Xu G, Wang X, Deng C, Teng X, Suuronen E, Shen Z, et al. Injectable biodegradable hybrid hydrogels based on thiolated collagen and oligo(acryloyl carbonate)-poly(ethylene glycol)-oligo(acryloyl carbonate) copolymer for functional cardiac regeneration. Acta Biomater. 2015;15:55-64 pubmed publisher
Zhao L, Yang G, Zhao X. Rho-associated protein kinases play an important role in the differentiation of rat adipose-derived stromal cells into cardiomyocytes in vitro. PLoS ONE. 2014;9:e115191 pubmed publisher
Yang L, Liu J, Bai X, Fan L, Han F, Jia W, et al. Acute downregulation of miR-155 at wound sites leads to a reduced fibrosis through attenuating inflammatory response. Biochem Biophys Res Commun. 2014;453:153-9 pubmed publisher
Tang J, Huang H, Ji X, Zhu X, Li Y, She M, et al. Involvement of IL-13 and tissue transglutaminase in liver granuloma and fibrosis after schistosoma japonicum infection. Mediators Inflamm. 2014;2014:753483 pubmed publisher
Tao H, Yang J, Chen Z, Xu S, Zhou X, Zhan H, et al. DNMT3A silencing RASSF1A promotes cardiac fibrosis through upregulation of ERK1/2. Toxicology. 2014;323:42-50 pubmed publisher
You H, Wei L, Sun W, Wang L, Yang Z, Liu Y, et al. The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats. Int J Mol Med. 2014;34:92-102 pubmed publisher
Liu T, Zeng Z, Liu Y, Wang J, Maitz M, Wang Y, et al. Surface modification with dopamine and heparin/poly-L-lysine nanoparticles provides a favorable release behavior for the healing of vascular stent lesions. ACS Appl Mater Interfaces. 2014;6:8729-43 pubmed publisher
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Liu X, Qian L, Nan H, Cui M, Hao X, Du Y. Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis. Exp Ther Med. 2014;7:669-674 pubmed
Gu G, Chen T, Zhou H, Sun K, Li J. Role of Wnt/?-catenin signaling pathway in the mechanism of calcification of aortic valve. J Huazhong Univ Sci Technolog Med Sci. 2014;34:33-36 pubmed publisher
Fan L, Hu K, Ji K, Sun Q, Xiong J, Yang L, et al. Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons. In Vitro Cell Dev Biol Anim. 2014;50:304-12 pubmed publisher
Yu K, Zhu P, Dong Q, Zhong Y, Zhu Z, Lin Y, et al. Thymic stromal lymphopoietin attenuates the development of atherosclerosis in ApoE-/- mice. J Am Heart Assoc. 2013;2:e000391 pubmed publisher
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image

image 1 :

Figure 1. IHC analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- Alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4Â°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1072) with DAB as the chromogen.

image 2 :

Figure 2. Western blot analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, . Lane 2: human placenta tissue lysates, . Lane 3: rat stomach tissue lysates,. Lane 4: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Smooth Muscle Actin antigen affinity purified monoclonal antibody (Catalog # MA1106) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha-Smooth Muscle Actin at approximately 43KD. The expected band size for Alpha-Smooth Muscle Actin is at 43KD.

product information

SKU :

MA1106

Product Name :

Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4)

Price :

315 USD

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

1A4

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Chicken

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.1-0.5µg/ml, Human, mouse, rat, By Heat. Immunohistochemistry (Frozen Section), 0.1-0.5µg/ml, Human, mouse, rat Western blot, 2µg/ml, Human, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) catalog # MA1106. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

ACTN

Uniprot ID :

P62738

Immunogen :

N-terminal synthetic decapeptide of alpha-smooth muscle actin.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Actin, aortic smooth muscle

Synonyms :

Actin, aortic smooth muscle; Alpha-actin-2; Acta2; Actsa, Actvs;

Protein Name :

Actin, aortic smooth muscle

Molecular Weight :

42009 MW

Protein Function :

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular Localization :

Cytoplasm, cytoskeleton.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Background :

Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

Research Category :

Cytoskeleton / Ecm, Microfilaments, Signal Transduction

more info or order :

Boster product webpage