Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) | Boster MA1105 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)

catalog :

MA1105

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

100/2

The same clone is also sold as:

MilliporeSigma: A4325
Santa Cruz Biotechnology: sc-58215

reactivity :

human, rat

application :

western blot

product information

SKU :

MA1105

Product Name :

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

100/2

Host :

Mouse

Reactivity :

Human, Rat

Predicted Reactivity :

Monkey

Application(s) :

Application Details :

Western blot, 2-4µg/ml, Human, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

AP2A1

Uniprot ID :

D3ZUY8

Immunogen :

AP-2 adaptor polypeptides from bovine brain.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

adaptor-related protein complex 2, alpha 1 subunit

Synonyms :

Adaptor protein complex AP-2, alpha 1 subunit (Predicted) ; Protein Ap2a1 ; Ap2a1 ; Ap2a1_predicted ; rCG_54084 ;

Protein Name :

AP-2 complex subunit alpha-1

Molecular Weight :

107675 MW

Protein Function :

AP2A1: Component of the adaptor protein complex 2 (AP-2).Adaptor protein complexes function in protein transport viatransport vesicles in different membrane traffic pathways. Adaptorprotein complexes are vesicle coat components and appear to beinvolved in cargo selection and vesicle formation. AP-2 isinvolved in clathrin-dependent endocytosis in which cargo proteinsare incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with theearly endosome. The clathrin lattice serves as a mechanicalscaffold but is itself unable to bind directly to membranecomponents. Clathrin-associated adaptor protein (AP) complexeswhich can bind directly to both the clathrin lattice and to thelipid and protein components of membranes are considered to be themajor clathrin adaptors contributing the CCV formation. AP-2 alsoserves as a cargo receptor to selectively sort the membraneproteins involved in receptor-mediated endocytosis. AP-2 seems toplay a role in the recycling of synaptic vesicle membranes fromthe presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi)and [ED]-X-X-X-L-[LI] endocytosis signal motifs within thecytosolic tails of transmembrane cargo molecules. AP-2 may alsoplay a role in maintaining normal post-endocytic traffickingthrough the ARF6-regulated, non-clathrin pathway. The AP-2 alphasubunit binds polyphosphoinositide-containing lipids, positioningAP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocyticaccessory proteins. The AP-2 alpha and AP-2 sigma subunits arethought to contribute to the recognition of the [ED]-X-X-X-L-[LI]motif (By similarity).

Subcellular Localization :

AP2A1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV. Ap2a1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.

Tissue Specificity :

AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Sequence Similarities :

Belongs to the adaptor complexes large subunit family.

Background :

Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Research Category :

Adapters, Signal Transduction, Transmembrane