Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) | Boster MA1104 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)

catalog :

MA1104

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EA-53

The same clone is also sold as:

MilliporeSigma: A7732, A7811
OriGene: DM227-05, DM227
Abcam: ab9465
Invitrogen: MA5-49311

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1104

Product Name :

Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

EA-53

Host :

Mouse

Reactivity :

Human, Mouse, Rabbit, Rat

Predicted Reactivity :

Monkey, Rabbit

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, mouse, rat, rabbit, By Heat. Immunohistochemistry (Frozen Section), 1-2µg/ml, Human, mouse, rat, rabbit Western blot, 0.5-1µg/ml, Human, mouse, rat, rabbit.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) catalog # MA1104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

ACTN2

Uniprot ID :

D3ZCV0

Immunogen :

Rabbit skeletal alpha-actinin.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

actinin, alpha 2

Synonyms :

Protein Actn2 ; RCG30552 ; Actn2 ; rCG_30552 ;

Protein Name :

Alpha-actinin-2

Molecular Weight :

103834 MW

Protein Function :

ACTN2: F-actin cross-linking protein which is thought to anchoractin to a variety of intracellular structures. This is a bundlingprotein.

Subcellular Localization :

ACTN2: Cytoplasm, myofibril, sarcomere, Z line Actn2: Cytoplasm, myofibril, sarcomere, Z line

Tissue Specificity :

ACTN2: Expressed in both skeletal and cardiac muscle.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the alpha-actinin family.

Background :

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.

Research Category :

Actin Binding Proteins, Actin Etc, Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction