Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1) | Boster MA1103 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)

catalog :

MA1103

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

hVIN-1

The same clone is also sold as:

Novus Biologicals: NB600-1293
Abnova: MAB1490
MilliporeSigma: V9264, V9131

reactivity :

human, mouse, rat, chicken

application :

western blot, immunocytochemistry, immunohistochemistry - frozen section

product information

SKU :

MA1103

Product Name :

Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

hVIN-1

Host :

Mouse

Reactivity :

Chicken, Human, Mouse, Rat

Predicted Reactivity :

Bovine, Rabbit

Application(s) :

IF, IHC-F, ICC, WB

Application Details :

Immunohistochemistry (Frozen Section), 2-4µg/ml, Human, chicken, mouse, rat Western blot, 1-2µg/ml, Human, chicken, mouse, rat.Immunocytochemistry/Immunofluorescence, 2µg/ml, Human

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1) catalog # MA1103. Tested in IF, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

VCL

Uniprot ID :

P85972

Immunogen :

Human vinculin, purified from uterus.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Vinculin

Synonyms :

Vinculin ; Metavinculin ; Vcl ;

Protein Name :

Vinculin

Molecular Weight :

116615 MW

Protein Function :

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).

Subcellular Localization :

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.

Tissue Specificity :

VCL: Metavinculin is muscle-specific.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Sequence Similarities :

Belongs to the vinculin/alpha-catenin family.

Background :

Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.

Research Category :

Actin Binding Proteins, Actin Etc, Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction