Anti-Vimentin Antibody (Monoclonal, V9) | Boster MA1102 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Vimentin Antibody (Monoclonal, V9)

catalog :

MA1102

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

V9

The same clone is also sold as:

Abcam: ab195878, ab8069, ab195877
Dako: M0725
MilliporeSigma: V6630, V6389
Santa Cruz Biotechnology: sc-6260, sc-6260 AC
Cell Signaling Technology: 49636
OriGene: TA355360, BM5050S, BM5050P, TA327753
Cell Marque: 347M
MyBioSource: MBS370116
Novus Biologicals: NBP1-97670
Invitrogen: 14-9897-80, 42-9897-82, 11-9897-82, 41-9897-82, 14-9897-37, MA5-11880, MA5-11883, 50-9897-80, 14-9897-82, MA5-11883-A555, 11-9897-80, MA5-11883-A488, 50-9897-82, 41-9897-80, MA5-11883-A647

reactivity :

human, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

SKU :

MA1102

Product Name :

Anti-Vimentin Antibody (Monoclonal, V9)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

Host :

Mouse

Reactivity :

Human, Pig, Rabbit, Rat

Predicted Reactivity :

Monkey

Application(s) :

IF, IHC-P, WB

Application Details :

Western blot, 0.5-1µg/ml, Human, rabbit, pig, rat. Immunohistochemistry (Paraffin-embedded Section), 1.5-2.5µg/ml, Human, rabbit, pig, rat, By Heat. Immunofluorescence, 5µg/ml, Human

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Vimentin Antibody (Monoclonal, V9) catalog # MA1102. Tested in IF, IHC-P, WB applications. This antibody reacts with Human, Pig, Rabbit, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

VIM

Uniprot ID :

P31000

Immunogen :

Pig eye lens vimentin.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Vimentin

Synonyms :

Vimentin; Vim;

Protein Name :

Vimentin

Molecular Weight :

53733 MW

Protein Function :

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Subcellular Localization :

Nucleus matrix. Cytoplasm.

Tissue Specificity :

VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Sequence Similarities :

Belongs to the intermediate filament family.

Background :

The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

Research Category :

Neuroscience, Signal Transduction, Stem Cells, Tumor Biomarkers