Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)

catalog :

MA1088

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SH-B1

The same clone is also sold as:

Invitrogen: MA5-32985
Abcam: ab218957
OriGene: DM111-05, DM111
MilliporeSigma: S2532
Abnova: MAB12455, MAB3572

reactivity :

human, rat

application :

immunohistochemistry

product information

SKU :

MA1088

Product Name :

Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

SH-B1

Host :

Mouse

Reactivity :

Human, Rabbit, Rat

Predicted Reactivity :

Horse, Monkey, Rabbit

Application(s) :

IHC

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, rabbit, rat, By Heat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-S-100 (Beta-subunit) S100b Antibody (Monoclonal, SH-B1) catalog # MA1088. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

S100B

Uniprot ID :

P04631

Immunogen :

Bovine brain S-100b.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Protein S100-B

Synonyms :

Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b;

Protein Name :

Protein S100-B

Molecular Weight :

10744 MW

Protein Function :

Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

Subcellular Localization :

Cytoplasm. Nucleus.

Tissue Specificity :

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Sequence Similarities :

Belongs to the S-100 family.

Background :

S100 protein is a 21,000-Da component first isolated from brain by Moore (1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin (Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a (0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.

Research Category :

Cell Biology, Cell Cycle, Cell Differentiation, Epigenetics and Nuclear Signaling, Neural Stem Cells, Stem Cells