Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26) | Boster MA1082 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26)

catalog :

MA1082

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PCK-26

The same clone is also sold as:

Invitrogen: MA1-25027

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1082

Product Name :

Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

PCK-26

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, mouse, rat, By Heat. Immunohistochemistry (Frozen Section), 1-2µg/ml, Human, mouse, rat Western blot, 0.5-1µg/ml, Human, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26) catalog # MA1082. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

Krt8

Uniprot ID :

Q10758

Immunogen :

Cytokeratin from human epidermis.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Keratin, type II cytoskeletal 8

Synonyms :

Keratin, type II cytoskeletal 8; Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8; Krt8; Krt2-8;

Protein Name :

Keratin, type II cytoskeletal 5

Molecular Weight :

54019 MW

Protein Function :

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Subcellular Localization :

Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.

Tissue Specificity :

Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the intermediate filament family.

Background :

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Research Category :

Class I, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Keratin, Signal Transduction