Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

catalog :

MA1081

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2

reactivity :

human, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1081

Product Name :

Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2

Host :

Mouse

Reactivity :

Human, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.1-0.5µg/ml, Human, rat, By Heat. Immunohistochemistry (Frozen Section), 0.1-0.5µg/ml, Human, rat Western blot, 1-2µg/ml, Human, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) catalog # MA1081. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

KRT1

Uniprot ID :

P04264

Immunogen :

mixture of several monoclonal cytokeratin clones.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a/ IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Keratin, type II cytoskeletal 1

Synonyms :

Keratin, type II cytoskeletal 1; 67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1; KRT1; KRTA;

Protein Name :

Keratin, type II cytoskeletal 1

Molecular Weight :

66039 MW

Protein Function :

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

Subcellular Localization :

Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.

Tissue Specificity :

The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the intermediate filament family.

Background :

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Research Category :

Cytoskeleton / Ecm, Intermediate Filaments, Microtubules, Signal Transduction