Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) | Boster MA1080 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10)

catalog :

MA1080

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PXC-10

The same clone is also sold as:

Invitrogen: MA1-24952
Abnova: MAB1478

reactivity :

human, mouse, rat, chicken

application :

western blot, immunocytochemistry

product information

SKU :

MA1080

Product Name :

Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

PXC-10

Host :

Mouse

Reactivity :

Chicken, Human, Mouse, Rat

Predicted Reactivity :

Canine, Horse, Rabbit

Application(s) :

ICC, WB

Application Details :

Immunocytochemistry , 1µg/ml, Human, mouse, rat, chicken Western blot, 1-2µg/ml, Human, mouse, rat, chicken.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) catalog # MA1080. Tested in ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

PXN

Uniprot ID :

Q66H76

Immunogen :

C-terminal part of recombinant chicken paxillin (amino acids 305-559).

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Paxillin

Synonyms :

Paxillin; Pxn;

Protein Name :

Paxillin

Molecular Weight :

64019 MW

Protein Function :

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Subcellular Localization :

Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity).

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Sequence Similarities :

Belongs to the paxillin family.

Background :

The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.

Research Category :

Adapters, Cancer, Cytoplasmic, Cytoskeleton / Ecm, Extracellular Matrix, Signal Transduction, Structures