Anti-P53 Tp53 Monoclonal Antibody | Boster MA1078 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-P53 Tp53 Monoclonal Antibody

catalog :

MA1078

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BP53-12

The same clone is also sold as:

BioLegend: 628202, 628201
Rockland Immunochemicals: 200-301-174
Exbio: 1F-114-C025, 11-114-C025, 1F-114-C100, 11-114-C100
Santa Cruz Biotechnology: sc-263
OriGene: AM10034FC-S, BM4079S, TA327920, BM4079F, AM10034FC-N, AM10034PU-N, AM10034PU-S
Abnova: MAB4528, MAB11254
Invitrogen: MA1-19055, MA1-19551
Novus Biologicals: NBP2-33074, NBP2-29453
US Biological: 138582

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1078

Product Name :

Anti-P53 Tp53 Monoclonal Antibody

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

BP53-12

Host :

Mouse

Reactivity :

Human

Application(s) :

IHC, ICC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-2µg/ml, Human, By Heat. Immunocytochemistry, 1µg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-2µg/ml, Human Western blot, 0.5-1µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-P53 Tp53 Monoclonal Antibody catalog # MA1078. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

TP53

Uniprot ID :

P10361

Immunogen :

Recombinant human wild-type p53 protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Cellular tumor antigen p53

Synonyms :

Cellular tumor antigen p53; Tumor suppressor p53; Tp53; P53;

Protein Name :

Cellular tumor antigen p53

Molecular Weight :

43451 MW

Protein Function :

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.

Subcellular Localization :

Cytoplasm. Nucleus. Endoplasmic reticulum. Mitochondrion matrix. Interaction with BANP promotes nuclear localization. Translocates to mitochondria upon oxidative stress (By similarity).

Tissue Specificity :

TP53: Ubiquitous. Isoforms are expressed in a widerange of normal tissues but in a tissue-dependent manner. Isoform2 is expressed in most normal tissues but is not detected inbrain, lung, prostate, muscle, fetal brain, spinal cord and fetalliver. Isoform 3 is expressed in most normal tissues but is notdetected in lung, spleen, testis, fetal brain, spinal cord andfetal liver. Isoform 7 is expressed in most normal tissues but isnot detected in prostate, uterus, skeletal muscle and breast.Isoform 8 is detected only in colon, bone marrow, testis, fetalbrain and intestine. Isoform 9 is expressed in most normal tissuesbut is not detected in brain, heart, lung, fetal liver, salivarygland, breast or intestine.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Belongs to the p53 family.

Background :

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Research Category :

Apoptosis, Cell Biology, Intracellular, P53 Pathway