Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) | Boster MA1076 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)

catalog :

MA1076

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-72

The same clone is also sold as:

Santa Cruz Biotechnology: sc-56338

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

SKU :

MA1076

Product Name :

Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

DCS-72

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

ICC, WB

Application Details :

Immunocytochemistry , 1µg/ml, Human, mouse, rat Western blot, 2-4µg/ml, Human, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) catalog # MA1076. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CDKN1B

Uniprot ID :

O08769

Immunogen :

recombinant rodent p27Kip1 protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

cyclin-dependent kinase inhibitor 1B(p27, Kip1)

Synonyms :

Cyclin dependent kinase inhibitor ; Cyclin kinase inhibitor ; Cyclin-dependent kinase inhibitor 1B ; P27 kip1 ; P27kip1 ; Protein Cdkn1b ; Cdkn1b ; p27Kip1 ; rCG_29991 ;

Protein Name :

Cyclin-dependent kinase inhibitor 1B

Molecular Weight :

22139 MW

Protein Function :

CDKN1B: Important regulator of cell cycle progression. Involvedin G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2complexes. Forms a complex with cyclin type D-CDK4 complexes andis involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or anactivator of cyclin type D-CDK4 complexes depending on itsphosphorylation state and/or stoichometry.

Subcellular Localization :

CDKN1B: Nucleus. Cytoplasm. Endosome Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates tothe cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results intranslocation to the cytoplasm and cell cycle progression.Phosphorylation on Ser-10 facilitates nuclear export. Translocatesto the nucleus on phosphorylation of Tyr-88 and Tyr-89.Colocalizes at the endosome with SNX6; this leads to lysosomaldegradation (By similarity). Cdkn1b: Nucleus. Cytoplasm. Endosome. Note: Nuclearand cytoplasmic in quiescent cells. AKT- or RSK-mediatedphosphorylation on Thr-197, binds 14-3-3, translocates to thecytoplasm and promotes cell cycle progression. Mitogen-activatedUHMK1 phosphorylation on Ser-10 also results in translocation tothe cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus onphosphorylation of Tyr-88 and Tyr-89 (By similarity). Colocalizesat the endosome with SNX6; this leads to lysosomal degradation.

Tissue Specificity :

CDKN1B: Expressed in all tissues tested. Highestlevels in skeletal muscle, lowest in liver and kidney.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Sequence Similarities :

Belongs to the CDI family.

Background :

P27 (kip1), also known as cyclin dependent kinase inhibitor 1B (CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B (p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27 (kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.

Research Category :

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling