Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) | Boster MA1074 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)

catalog :

MA1074

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-50

The same clone is also sold as:

Abnova: MAB1472
MyBioSource: MBS531744, MBS830658

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1074

Product Name :

Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

DCS-50

Host :

Mouse

Reactivity :

Human

Application(s) :

IHC, ICC, WB

Application Details :

Immunocytochemistry , 1µg/ml, HumanImmunohistochemistry (Frozen Section), 1-2µg/ml, Human Western blot, 0.5-1µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) catalog # MA1074. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CDKN2A

Uniprot ID :

Q9R0Z3

Immunogen :

Recombinant human p16 protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Cyclin-dependent kinase inhibitor 2A

Synonyms :

Cyclin-dependent kinase inhibitor 2A ; Cyclin-dependent kinase 4 inhibitor A; CDK4I; p16-INK4a; p16; p16-INK4; Cdkn2a ; P16ink4a ;

Protein Name :

Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3

Molecular Weight :

17366 MW

Protein Function :

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity).

Subcellular Localization :

Cytoplasm. Nucleus.

Tissue Specificity :

Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Sequence Similarities :

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Background :

p16 (INK4A), also known as cyclin-dependent kinase inhibitor 2A (CDKN2A), or multiple tumor suppressor 1 (MTS1). The p16 gene (CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.

Research Category :

Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, P53 Pathway, Transcription, Tumor Suppressors